Self-Excited Cooperative Motions of Cancer and Endothelium Cell Mixtures
Yi-Teng Hsiao (蕭翌登)1*, Hsiang-Ying Chen (陳香穎)1, Shu-Chen Liu (劉淑貞)2, Tien Hsu (徐沺)2, Wei-Yen Woon (溫偉源)1, Lin I (伊林)1
1Department of Physics, National Central University, Taoyuan City, Taiwan
2Department of Biomedical Sciences and Engineering, National Central University, Taoyuan City, Taiwan
* presenting author:Yi-Teng Hsiao, email:tonyhsian@gmail.com
Cells exhibit cooperative motion through the interplay of active driving, adhesion, and mutual coupling in many biological processes, such as embryogenesis, tumorigenesis and wound healing. Past studies focused on the collective motion with increasing packing density of single type confluent cell monolayer. In this work, collective motions of the monolayer endothelium-cancer cell mixtures are experimentally investigated in situ. We use the endothelial cells (ECs) monolayer as the base system, and cancer cells (wild type bone marrow metastasis nasopharyngeal carcinoma cell) which have higher motility than that of ECs as additives to ECs to form the binary cell mixture. It is found that the EC only system exhibits solid like behaviors with short-time self-excited cooperative rocking motions. Cancer cell additives tend to aggregate in the form of liquid like clusters. Their presence also causes the transition of ECs from the solid-like state to the liquid-like state.


Keywords: Cooperative Motion, Cancer cell, Endothelial cell, Binary mixture, Self-excited cooperative rocking motion