Enhanced Performance of Charge Detection Rectilinear Ion Trap Mass Spectrometer (Cd-Rit Ms) with Dipolar Resonant Excitation
Avinash A. Patil1*, Chen-Wei Lee1, Ming-An Wu1, Szu-Wei Chou1, Wen-Ping Peng1
1Physics, National Dong Hwa University, Shoufeng,Hualien, Taiwan
* presenting author:Avinash Adhikrao Patil, email:avi.smash4@gmail.com
In macro-molecule ion/ion chemistry, large organic ion-molecule reactions can have several channels that lead to a multitude of product ion species and there is huge demand to improve the resolution of the mass analysis and quantify the amount of trapped ions. Especially, top-down proteomic and metabolomic analyses become critically important for the analysis of biomedical samples. In our previous study, high mass protein analysis with a charge detection rectilinear ion trap mass spectrometer (CD-RIT MS), a modified design of linear ion trap, showed that the ion number (by charge detection) and mass resolution of the analyzed proteins could be obtained with boundary ejection method. This study is to enhance the ion signal intensity and mass resolution of CD-RIT MS with the dipolar resonant excitation so that ions could be ejected at low q value with higher kinetic energy. Samples of C60 (m/z ~720), CsI clusters (m/z ~250-1000), cytochrome C (m/z ~12k) and myoglobin (m/z ~17k) were tested and measured. We found that the ion signal intensity of resonantly ejected ions was increased by a factor of 3-4 and mass resolution was improved by a factor of 2.

Keywords: Rectilinear Ion trap , Mass spectrometry, Protein , Dipolar Resonant Excitation, Charge Detector